While airborne transmission of viruses has long been a significant area of research, foot-and-mouth disease virus (FMDV) is relatively understudied in this regard. This is important as FMD has severe economic impacts; the 2001 outbreak cost the UK approximately £8 billion, in addition to the culling of 6.5 million animals. Although the primary mechanism of transmission is direct contact between infected cloven-hooved animals, a low probability but high consequence transmission mechanism is via aerosols. Aerosols can travel much further distances than larger droplets and therefore have the potential to circumvent quarantine zones, an important part of FMD control. This, combined with the fact that several outbreaks of FMD have likely resulted from aerosol transmission, demonstrates the importance of studying the conditions determining if infectivity is retained during aerosol transport.  This work studies FMDV survival in aerosols under a range of environmental conditions in high containment. Two complementary experimental methods were employed, 1) the use of microthreads, produced by winding natural spider silk onto frames and exposing them to FMDV in a controlled aerosol chamber; and 2) the CELEBS instrument (Controlled Electrodynamic Levitation and Extraction of Bioaerosols onto a Substrate), which allows individual virus-containing aerosols to be suspended under precisely controlled relative humidity or temperature. These methods will provide survival parameters for FMDV and allow strains to be compared. Results can determine if quarantine zones around infected farms are appropriate, account for the risk of aerosolised transmission, and ultimately better inform outbreak policy resulting in more effective control of FMD.